Re: Source for 99+% accuracy claim 24 November 2004
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Peter J Flegg,
Physician
Blackpool, UK FY3 8NR

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Re: Re: Source for 99+% accuracy claim

Some (slightly old) references on HIV ELISA assays, the first of which goes back to an evaluation of the first generation assays in 1986. (There are probably much more recent reviews available which include details of the latest assays which employ only recombinant or purified antigens, giving even higher specificities - I suggest Dr Da Prato hunts these down with PubMed or similar).

References:

Ward JW, Grindon AJ, Feorino PM, Schable C, Parvin M, Allen JR. Laboratory and epidemiologic evaluation of an enzyme immunoassay for antibodies to HTLV-III. JAMA. 1986;256:357-361.

Weber B, Moshtaghi-Boronjeni M, Brunner M, Preiser W, Breiner M, Doerr HW. Evaluation of the reliability of 6 current anti-HIV-1/HIV-2 enzyme immunoassays. J Virol Methods. 1995;55:97-104.

Steckelberg JM, Cockerill III FR. Serologic testing for human immunodeficiency virus antibodies. Mayo Clin Proc. 1988;63:373-380.

Centers for Disease Control and Prevention. Current trends update: serologic testing for antibody to human immunodeficiency virus. MMWR Morb Mortal Wkly Rep. 1988;39:833-840.

Proffitt MR, Yen-Lieberman B. Laboratory diagnosis of human immunodeficiency virus infection. Infect Dis Clin North Am. 1993;7:203- 219.

Cordes RJ, Ryan ME. Pitfalls in HIV testing: application and limitations of current tests. Postgrad Med. 1995;98:177-80, 185, 186, 189.

George JR, Schochetman G. Detection of HIV infection using serologic techniques. In: Schochetman G, George JR, eds. AIDS Testing: A Comprehensive Guide to Technical, Medical, Social, Legal and Management Issues. New York, NY: Springer Verlag; 1994:62-102.

Competing interests: None declared